12/29/2023 0 Comments Amplifx pcr closed triangle![]() Traditionally, species identification has been based on floral characters, leaf type, seed colour, pod angle and the relative positioning of opened and unopened buds. In addition, each Brassica species has evolved multiple morphotypes, including enlarged organs of stems and inflorescences, oilseeds, and even ornamental features. This potential for hybridization with wild relatives combined with the rich genetic diversity in non-domesticated forms of key crop species make Brassica a prominent feature of genebank collections worldwide.īrassica crops described by U’s triangle are close relatives that share many independently developed traits, such as heading leaves and enlarged roots. Several species within the broader Brassicaceae can also hybridize with important Brassica crop species, including the wild radishes ( Raphanus), woad ( Isatis) and white mustard ( Sinapsis). oleracea (C genome, n = 9) gave rise to the three allotetraploids, B. 1), in which hybridization between each pair of the three progenitor diploid species B. The genetic relationships among the six Brassica species are described by U’s triangle model (Fig. Within the Brassicaceae, six species from the genus Brassica produce numerous types of oilseeds, condiments and vegetables of global economic significance. Brassicaceae are readily distinguished from other flowering plant families by having a cruciform (cross-shaped) corolla, six stamens (the outer two shorter than the inner four), a capsule often with a septum and a pungent watery sap. The Brassicaceae family (mustards or crucifers) is the most species-rich member of the order Brassicales, comprising 3709 species and 338 genera, with 308 genera further assigned to 44 tribes. The MPCR assay can be readily implemented in any basic molecular laboratory and should prove useful for the management of Brassica germplasm collections in genebanks. ConclusionĪ cheap and fast multiplex PCR assay for identification of Brassica species in the triangle of U was developed and validated in this study. In addition, the MPCR was sensitive enough to detect seed contaminations in pooled seed samples of Brassica accessions. Further validation against 120 Brassica accessions previously genotyped showed that the MPCR is highly accurate and comparable to more advanced techniques such as SSR marker sequencing or the Illumina Infinium B. ResultsĪ multiplex polymerase chain reaction (MPCR) consisting of new and existing primers specific to the Brassica A, B and C genomes was able to reliably distinguish all six Brassica species in the triangle of U with 16 control samples of known species identity. A cheaper, faster and simpler method for Brassica species identification is described here. However, these methods are technically challenging, expensive and time-consuming, making them unsuitable for routine or rapid screening of Brassica accessions for germplasm management. Molecular genotyping based on simple sequence repeat (SSR) marker sequencing or the Illumina Infinium Brassica napus 60K single nucleotide polymorphism (SNP) array has been used to identify species and assess genetic diversity of Brassica collections. Consequently, a major issue for genebank collections is ensuring the correct identification of species. Extensive shared traits and diverse morphotypes among Brassica species make identification and classification based on phenotypic data alone challenging and unreliable, especially when dealing with large germplasm collections. The genetic relationships among the six Brassica species are described by U’s triangle model. Within the Brassicaceae, six species from the genus Brassica are widely cultivated throughout the world as oilseed, condiment, fodder or vegetable crops.
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